Figure 3.

Decreased viral load in tissues is associated with survival. Tissues (lung—left column, spleen—middle column and brain—right column) from four animals per group were collected 5 days after challenge. Tissues were homogenised and total RNA was extracted. TCID₅₀ equivalents were determined by quantitative reverse transcription PCR using an N gene-specific primer and probe set with a standard of known TCID₅₀ equivalents. Individual animals from each vaccination group (DMEM—top row, rVSV-EBOV-GP—middle row and rVSV-EBOV-GP-NiV-G—bottom row) are represented by individual dots, bars represent the mean of each group and error bars indicate s.e.m. Groups of animals that were protected by vaccination in the survival experiment are designated by (+), partially protected by (±) and groups not protected by (−). One-way analysis of variance with Tukey’s post tests was performed on each vaccination time point and significance is represented by asterisks (*P⩽0.05 and **P⩽0.01). Black asterisks denote significance between the vaccinated groups and DMEM controls, and grey asterisks represent differences between the vaccinated groups and vector controls.