Fig 6. LFA-1 is required for residence of Plasmodium specific T_RM cells in the liver.

(A) 2 × 10⁴ naïve CD45.1⁺ WT OT-I cells were co-transferred with 2 × 10⁴ naïve GFP⁺ Itgal^-/- OT-I cells to C57BL/6 1 day prior to immunization with 5 × 10⁴ P. berghei CS^5M sporozoites, at 1 week and 4 weeks post-immunization organs were harvested and the number and phenotype of transferred cells determined by flow cytometry. (B) Representative plots from a single mouse at each time-point showing the expansion of the different OT-I⁺ populations in the spleen and liver 1 and 4 weeks post immunization. (C) Summary data showing the overall ratio of Itgal^-/- (KO) to WT OT-I cells in the spleen and liver of mice (i) 1 week and (ii) 4 weeks post-immunization. (D) Representative flow cytometry plots showing the T_RM phenotype of WT and Itgal^-/- OT-I cells in the spleen and liver of a single animal 4 weeks post immunization. (E) Summary data the showing percentage of WT and Itgal^-/- OT-1 cells that are T_RM in (i) the spleen and (ii) livers 4 weeks post immunization. (F) Summary data showing the overall ratio of Itgal-/- (KO) to WT OT-I cells that are (i) T_RM and (ii) non T_RM. (G) Summary data of the overall ratio of Itgal^-/- (KO) to WT OT-I cells in different organs of mice analyzed 4 weeks post-immunization; bars show means and SD. All data are pooled from 9 mice in 2 independent experiments. Panels C and F were analyzed using linear mixed models with mouse as a random effect and organ as the fixed effect. Panel E was analyzed similarly to C but with genotype as the fixed effect. Panel G was analyzed similarly to C but with experiment included as a random effect.