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. Author manuscript; available in PMC: 2017 Nov 11.
Published in final edited form as: ACS Infect Dis. 2016 Aug 15;2(11):800–806. doi: 10.1021/acsinfecdis.6b00106

Figure 2.

Figure 2

cyp128 and stf 3 are required for SMK production. (A) Mass spectra of TLE from M. smegmatis strains expressing SMK biosynthetic genes. Only strains containing both cyp128 and stf 3 produced SMK. The introduction of each of gene individually is not sufficient for SMK biosynthesis. (B) Mass spectra of TLE from M. tuberculosis WT and SMK deletion mutants. The deletion of cyp128 from M. tuberculosis H37Rv (Δcyp128) resulted in a loss of SMK production similar to that of the previously characterized stf 3 deletion mutant (Δstf 3). Complementation of Δcyp128 (Δcyp128::cyp128) restores SMK production. A representative scan from one experiment of FT-ICR mass spectra in region m/z 881– 882 is shown.