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. 2017 May 26;102(2):507–516. doi: 10.1189/jlb.3A1216-538RR

Figure 1. Neutrophil lung infiltration and inflammation correlates inversely with circulatory ST6Gal-1.

Figure 1.

WT, dP1, B16-cntl, and B16-S6G mice were subjected to 50 μg of LPS by oropharyngeal instillation. At 18 h, the BALF was collected and the neutrophils counted. Serum samples were collected for enzyme analysis. (A) Sialyltransferase activities in the sera were measured by following the transfer of CMP-[3H]Sia to Galβ1–4GlcNAc-O-Bn (LacNAc). The α2,6-Sia product formed by ST6Gal-1 is shown (n = 3). ***P < 0.001. (B) Total number of neutrophils recovered from the BALF of LPS-induced animals (n = 3). *P < 0.05. (C and D) B16-cntl (C) and B16-S6G (D) show a decreased number of infiltrated neutrophils at 18 h. Arrows: infiltrating neutrophils around the bronchiole and within the bronchiolar epithelium.