Skip to main content
. Author manuscript; available in PMC: 2017 Nov 29.
Published in final edited form as: Nat Med. 2017 May 29;23(7):885–889. doi: 10.1038/nm.4347

Fig. 2. TZM-bl cells are insensitive to infection by replication defective HIV-1.

Fig. 2

a) J-Lat clones 10.3, 9.2 and 8.4 were stimulated with PHA, serially diluted with uninfected CD8+ T cell-depleted PBMC and added to TZM-bl cells. β-gal activity was measured 48 h later. b) 8E5 cells were serially diluted with uninfected CD8+ T cell-depleted PBMC and added to TZM-bl cells. β-gal activity was measured 48 h later. c) Different p24 amounts of wild type (wt) HIV-1LAI, and a mutant virus containing the L289K mutation in reverse transcriptase that renders the enzyme defective, were added to TZM-bL cells and β-gal activity was measured 48 h later. Statistical comparison of wild type versus mutant HIV-1 β-gal activity was performed using a Student’s T test (*, P < 0.05).