Skip to main content
. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Neurochem Res. 2017 Apr 10;42(7):2033–2054. doi: 10.1007/s11064-017-2260-6

Fig. 7. UCHL1 inhibition prior to SE is associated with increased hippocampal cell death.

Fig. 7

(a) Hippocampal neuropathology was assessed at rostral, medial and ventral stereological levels following SE. (b) Representative examples of neurodegeneration (FJB staining) in the ipsilateral CA3 of the hippocampus in vehicle and UCHL1 inhibitor-treated mice at 24 h. (c) Semi-quantitative analysis of FJB counts 24 h after SE or receiving sham saline injection, on cryosections from LDN- and vehicle-treated mice (n = 6–10, one-way ANOVA with Tukey’s post-hoc test). (d) Analysis of damage for each stereotaxic level, confirming strongest effect of LDN-7544 at ventral level following SE (n = 8 – 10, two-way ANOVA with Bonferroni post-hoc test) (e) TUNEL staining on ventral hippocampal sections confirming enhanced cell death in mice treated with UCHL1 inhibitor (n = 10, unpaired Student’s t-test, two-tailed). (f) Semi-quantitative analysis of MDM2 and p53 levels detected by immunoblotting, on samples 4 h after SE or sham injection, in mice receiving LDN-57444 or vehicle (n = 4 to 6,Mann-Whitney U or Kruskal-Wallis test with Dunn’s multiple comparison test). (g) Representative images of MDM2 and p53 blots. Data expressed as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. CA, cornu ammonis; DG, dentate gyrus; LDN, LDN-57444; VEH, vehicle