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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Neurochem Res. 2017 Apr 10;42(7):2033–2054. doi: 10.1007/s11064-017-2260-6

Fig. 8. UCHL1 downregulation in the hippocampus is post-transcriptional and is preceded by depletion of AsUchl1.

Fig. 8

(a) Schematic depiction of the genetic landscape of Uchl1, in which a partially overlapping antisense non-coding RNA (AsUchl1) also resides. Points of primer pair binding are shown (arrows). The regulatory SINEB2 region is also highlighted. (b) DNA agarose gels were run to ensure successful, specific amplification with primer pairs targeting AsUchl1 and Uchl1. Primers targeting the SINEB2 region were used in subsequent investigations. (c) Hippocampal subfield microdissection was carried out. (d, e) RT-qPCR was carried out for each subfield at 8 h (left) and 24 h (right) for (d) Uchl1 mRNA following 1 μg KA and (e) AsUchl1 lncRNA following 0.3 μg KA (n = 4 to 6, Mann-Whitney U test, two-tailed). Data expressed as mean ± SEM, *P < 0.05. CA, cornu ammonis; CON, control; DG, dentate gyrus; INJ, injury