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. Author manuscript; available in PMC: 2018 Sep 10.
Published in final edited form as: J Pharm Biomed Anal. 2017 Jan 11;144:12–24. doi: 10.1016/j.jpba.2017.01.026

Figure 7.

Figure 7

Scheme for (a) the injection of a drug/protein sample onto an HSA microcolumn, and (b) use of ultrafast affinity extraction with this sample and microcolumn to determine the association equilibrium constant (Ka) or global affinity (nKa′) for the drug-protein interaction in solution (middle panel) or the dissociation rate constant (kd) for the drug from the soluble drug-protein complex (bottom panel). Reproduced with permission from Ref. [162].