Figure 7.
Scheme for (a) the injection of a drug/protein sample onto an HSA microcolumn, and (b) use of ultrafast affinity extraction with this sample and microcolumn to determine the association equilibrium constant (Ka) or global affinity (nKa′) for the drug-protein interaction in solution (middle panel) or the dissociation rate constant (kd) for the drug from the soluble drug-protein complex (bottom panel). Reproduced with permission from Ref. [162].