Figure 4.

H3K4 Demethylation in Donor Nuclei Improves the Development of NT Embryos

(A) IVF, NT(Kdm5b^ci), and NT(Kdm5b^wt) embryos at the gastrula and tadpole stages.

(B) The development of IVF, NT(Kdm5b^ci), and NT(Kdm5b^ci) gastrula embryos (stage 10.5) was followed until feeding tadpole stage (green lines and black solid line, respectively). Black dashed lines indicate the developmental potential of NT embryos generated from uninjected blastula stage or neurula stage endoderm nuclei (data from Gurdon, 1960). y axis is the percentage of gastrula embryos reaching the indicated stages.

(C) Kdm5b^WT expression in the donor cell improves the development of NT embryos to the feeding tadpole stage. Bar graph showing the percentage of cleaved gastrula embryos reaching the feeding tadpole stage (^∗p value = 0.0007, paired t test, one-tailed). Data are presented as mean ± SEM.

See also Figure S4 and Table S1.