Abstract
Electrophoretic and immunological properties of the soluble root protein complement of 6 Medicago sativa L. genotypes in the cold hardened and non-hardened physiological condition were compared. These 6 genotypes were chosen to represent a range of abilities to survive exposure to subfreezing temperatures when in the cold hardened condition.
A zone of highly charged and/or low molecular weight protein components were found to be more prevalent in the protein complements of the cold-hardened material than the non-hardened material.
Immunodiffusion plate tests were not so definitive as the electrophoretic patterns for identifying the genotypes or physiological conditions, but did corroborate the electrophoretic interpretations.
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