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. 2017 Jul 11;7:5117. doi: 10.1038/s41598-017-05383-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of mechanical properties of polyacrylamide gels measured with conventional procedure and with SNAP. (A) Elastic moduli of eight gels prepared centrally in Bremen and measured from the different participating labs. Each diamond marker indicates the mean value of the elastic modulus extracted from each force map; data were acquired and analysed using the conventional AFM procedure. Different marker colours stand for different AFMs from several manufacturers: JPK (NanoWizard III), Bruker (Catalyst, Nanoscope and Multimode), Park System (XE120) and Asylum (MFP3D). (B) Same eight polyacrylamide gels were measured in Bremen and elastic moduli were evaluated applying the standardized nanomechanical AFM procedure (SNAP). Each circle marker indicates the mean value of the elastic moduli extracted from a force map (made of 100 force curves). On each gel three force maps were recorded. Bars represent standard deviations.