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. 2017 Jul 11;7:5117. doi: 10.1038/s41598-017-05383-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SNAP reduces the variability of mechanical measurements on the same gel in different locations. Elastic moduli of the same gel measured with three different instruments using the same kind of colloidal probes. The data were then analysed with different methods numbered from 1 to 4. In 1 to 3 ones, the deflection sensitivity was calibrated with a force curve on a stiff substrate and the force constant was determined from the analysis of the thermal with the AFM software (1); from the analysis of the thermal with the JAVA applet (2) and from the vibrometer measurements (3). In method 4, SNAP was applied: the force constant was measured using the vibrometer and deflection sensitivity was re-calibrated with the correction factor λ.