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. 2017 Jul 11;7:5116. doi: 10.1038/s41598-017-05379-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MD1 regulates TLR4 signalling and CaMKII signalling in response to pressure overload. Western blot analysis of WT and MD1-KO left-ventricle samples at 4 weeks after surgery for (A) RP105, MD2, and TLR4 expression; (B) ox-CaMKII and total CaMKII expression, and phosphorylation of CaMKIIβ + γ + δ at Thr287. Left: representative immunoblots; right: quantitative results. Data were normalised to GAPDH. Numbers of mice per group are shown inside bars. *P < 0.05 vs. WT-Sham, ^# P < 0.05 vs. WT-AB.