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. 2017 Jul 11;7:5089. doi: 10.1038/s41598-017-04800-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Tal7 suppresses avrXa7-Xa7 mediated defense. (a) Diagram showing the RVDs of Tal7 and AvrXa7 and schematic construction of Tal7∆NA. (b) Tal7 suppressesthe avrXa7-Xa7 interaction in rice. Strain YNB0-17 harboring pHM1, avrXa7, avrXa7 + tal7, and avrXa7 + tal7∆NA were infiltrated into rice seedlings of IRBB7(Xa7) with needleless syringes. The T3SS-deficient strain of RS105, R∆hrcV (Supplemental Table S1), containing avrXa7 + tal7, was used as a control. Leaves were scored for water-soaked symptoms or the HR at 3 dpi and were designated as susceptible or resistant, respectively. Leaves were photographed 3 dpi. (c) Secretion of AvrXa7-Flag and (d) Tal7-c-Myc by strain YNB0-17 harboring the constructs mentioned in panel b. Bacterial cells containing p707 or p707ΔNA (Supplemental Table S1) were induced in XOM3 medium as described in Methods. Bacterial supernatants (SN) and total extracts (TE) were analyzed by SDS-PAGE, transferred to polyvinylidene difluoride membranes, and used for immunoblotting with anti-FLAG (c) or anti-C-Myc (d) as the primary antibody. The experiments were repeated three times, and similar results were obtained each time.