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. 2017 Jul 11;7:5113. doi: 10.1038/s41598-017-05140-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Hydrolysis of colloidal chitin by EcChi1. The EcChi1 (0.8 μM) was incubated with 30 mg/ml of the substrate in 900 μL reaction mixture for different time periods from 0 to 720 min at 40 °C. The reaction products were analyzed by binary gradient HPLC. (A) The topmost profile shows a standard mixture of CHOS ranging from DP1 to DP6. The remaining profiles are the reactions at different incubation times. Control represents the substrate without EcChi1 while standard represents CHOS ranging from DP1 to DP6. (B) Overview of the quantifiable CHOS products generated during hydrolysis. Products were quantified from respective peak areas using standard calibration curves of CHOS ranging from DP1 to DP4.