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. 2017 Jul 11;7:5121. doi: 10.1038/s41598-017-04974-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Naringenin inhibits the activity of the human TPC2 channel. (a) Time course of current amplitude recorded in response to bath application of 330 nM PI(3,5)P₂ (upper black bar) before and after adding 500 μM Nar (upper blue bar). Each point represents the steady-state current at +40 mV. (b) Currents mediated by hTPC2 and induced by the application of 330 nM PI(3,5)P₂ in the cytosolic bath solution were recorded at +40 and −40 mV (shown in the upper voltage profile) in the absence or presence of 500 μM cytosolic Nar. For the sake of clarity, background currents in the absence of the phosphoinositide were directly subtracted. (c) Dose–response analysis of Nar inhibition. I_norm, the normalised current, was obtained by the ratio of currents recorded respectively in the presence and absence of cytosolic Nar. Both at +40 and −40 mV, normalised currents at different Nar concentrations were fitted with a Michaelis–Menten function (continuous lines). Data from 3 (Nar 33 μM), 6 (Nar 100 μM) and 9 (Nar 500 μM) different vacuoles were shown as mean ± s.e.m. Values at positive and negative voltages, at a defined Nar concentration, were not significant (P > 0.2); values at different Nar concentrations and at a defined voltage (+40 or −40 mV), were statistically significant (at least P < 0.01).