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. 2017 Jul 11;7:5121. doi: 10.1038/s41598-017-04974-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Naringenin impairs in vivo vascularization induced by VEGF in C57BL/6 mice. In vivo vessel formation was assessed after subcutaneous injection of 5 weeks old male/female C57BL/6 mice with Matrigel plugs containing either vehicle or VEGF or VEGF plus 1000 μM Nar. Five days after injection the mice were sacrificed and plug vascularization was evaluated both macroscopically, as shown in two representative images (a) and as hemoglobin content expressed as absorbance (OD)/1 g matrigel plug (b); values from three independent experiments are expressed as mean ± s.e.m. *P < 0.05. n = 14–15 plugs for each experimental condition.