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. 2017 Jul 11;7:5129. doi: 10.1038/s41598-017-05311-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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GTSE1 regulates EMT in vitro. (a) The protein levels of GTSE1, N-cadherin, β-catenin, and Snail were determined by western blotting in QGY-7703 and SMMC-7721 cells stably transfected with shGTSE1 or GTSE1 or control. Relative protein fold changes compared with the control are marked below. Original data are presented in Supplementary Fig. 4. (b) The mRNA expression levels of N-cadherin, β-catenin, and Snail were detected by qPCR in SMMC-7721. (c) The protein levels of 4EBP1, p-4EBP1 (T37/46), and p-4EBP1 (S65) were tested by western blotting in QGY-7703 and SMMC-7721. Original data are presented in Supplementary Fig. 5.