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. 2017 Jul 11;7:5147. doi: 10.1038/s41598-017-04367-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Response of C-20/A4 cells and AC to Ucn1 blockade. Cells were treated for 8 h with CP-154526, astressin 2B or in combination at indicated concentrations. (A) The effects on morphology of incubating C-20/A4 cells and AC with CP-154526 and/or astressin 2B (1–50 μM) was assessed using phase contrast microscopy. (B) Dose dependant effects of CP-154526 and/or astressin 2B on C-20/A4 cells. (C) AC cell death as determined by Annexin V/PI staining and flow cytometry. n = 3, error bars = SD *p < 0.05 and **p < 0.005 compared with untreated control cells.