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. 2017 Jul 11;7:5160. doi: 10.1038/s41598-017-04896-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In vitro proliferation of satellite cell-derived muscle precursors. Purified satellite cells were prepared by mechanically stripping isolated, intact muscle fibres. Clones were then assessed by plating at 0.5 cells/well in 96 well plates, and culturing for 7 days. Representative images of clones derived from 22 month old C5/B/l6 (A,B) and DBA/2 (C,D) mice. Quantification of cell numbers/clone in C57Bl/6 (C) and DBA/2 (F) mice. n = 23–170 clones per time point/strain/diet; for each, 3–4 mice from each sex were used. Data are presented as mean +/− SEM. ^#Represents significance between 22 month old mice, and their 12 month counterparts (of same sex, strain and diet).