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. 2017 Jul 11;7:5075. doi: 10.1038/s41598-017-04747-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of in vivo knockdown of Homer1 on neointima formation. Balloon-injured arteries were treated with AAV-shScrambled(-shScr) or AAV-shHomer1. (a) Representative immunofluorescence staining images of Homer1, STIM1, Orai1, Ki-67, and MHC with DAPI in injured (shScr- or shHomer1-treated) and uninjured arteries. (b) Quantification of neointimal area in shScr- and shHomer1-treated injured arteries. Neointima area in shScrambled (shScr)-treated arteries is set to 100%. *p < 0.05 or better compared to shScr. (c) Fluorescence Intensity Ratios of Homer1, Ki-67, and MHC. Intensities are normalized to DAPI (DNA fluorescence); AU, arbitrary units. *p < 0.05 or better compared to shScr or uninjured arteries in corresponding category (NI or M) and stain; ^#p < 0.05 compared to shScr in media. n = 5 for shScr-treated injured arteries; n = 6 for uninjured and shHomer1-treated injured arteries. NI = Neointima; M = Media; U = Uninjured; I = Injured.