Figure 3.

Metformin does not induce fission of the mitochondrial network, and mitochondrial swelling is partially dependent on AMPK. (A) LNCaP cells were treated with metformin for the indicated times, and Mfn2 expression was monitored at different time by immunoblotting. (B) LNCaP and DU145 cells were treated for 24 h with 5 mM metformin and stained with Tom20 for immunofluorescence analysis. Representative IF images are shown with the quantification of the morphology of the mitochondrial network. The data from the counting of at least 100 cells are shown. (C) LNCaP cells were treated with 5 mM metformin for 24 h in the presence or absence of 20 µM of compound C (CC). Representative electronic microscopy images are shown, and the graph represents the mean of the relative mitochondrial area. For each condition, 60 to 100 mitochondria from several cells were analyzed by EM, p < 0.001 (***).