Figure 2.

Some Zfh1 positive cells are not derived from cyst cell lineage when Abd-B was overexpressed in CySCs. (a-a”’) Representative testes in the lineage tracing experiments using the c587-Gal4^ts, uas-GFP control transgene showing GFP (green), Zfh1 (red) and Vasa (blue) after 20 days’ induction at 29 °C, in which the GFP colocalizes with Zfh1. (b-b”’) c587-Gal4^ts, uas-GFP > uas-Abd-B testes showing the GFP (green), Zfh1 (red) and Vasa (blue) after 20 days’ induction at 29 °C, (c-c”’) the detailed view of the white circled part in (b-b”’). Most of the cells in the yellow broken lines are stained with both Zfh1 (red) and Vasa (blue) signals, but not GFP, as the representative cells signed with the white arrows. Scale bar, 10 μm.