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. 2017 Jul 11;7:5096. doi: 10.1038/s41598-017-05028-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fitting of decay curves highlights distinction between targeting speed. (a) Averaged fluorescence intensity profiles for each group were fitted to two-term exponential functions. The calculated fast and slow decay curve half-lives were calculated and are show, along with the measured time for the curve to reach 1/e. The curve fitting precision is represented by the adjusted R2 value. (b) Individual fluorescence intensity decay curves were fitted to two-term exponential functions for each sample. KillerRed photobleaching follows this two-term decay curve extremely well, comprising a fast and a slow decay component. The half-life was calculated from the fast term of each fitted curve, and averaged for each group. Individual points are plotted, with error bar designating the 95% confidence intervals. **p < 0.01, ****p < 0.0001.