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. 2017 Jul 12;8:1229. doi: 10.3389/fpls.2017.01229

FIGURE 2.

FIGURE 2

Analysis of autophagic flu in GFP-ATG8a. Five-day-old seedlings of GFP-ATG8a were starved by liquid MS medium without sugar following stimulated by 1% D-glucose for 0, 0.5, and 2 h. (A) Equal amounts of protein extracted from the seedlings were used to SD-PAGE, followed by western blotting with anti-GFP and anti-plant-actin antibodies. (B) Quantification of changes in free GFP normalized with the expression of plant-actin. Asterisks indicate significant differences from starved seedlings treated with 1% D-glucose for 0 h at P < 0.05 or ∗∗P < 0.01. Error bar represent SD obtained from three independent replicates.