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. 2017 Jul 12;8:1229. doi: 10.3389/fpls.2017.01229

FIGURE 5.

FIGURE 5

Detection of AtRGS1-YFP protein. The seedlings of AtRGS1-YFP (A), atg2/AtRGS1-YFP (B), atg5/AtRGS1-YFP (C) grown in liquid MS medium, then starved for 2 h, followed by 1% D-glucose application for 0, 0.5, and 2 h. Then the expression of AtRGS1-YFP protein were analyzed with anti-YFP antibodies. Plant-actin was used as the uniform protein loading. (D) Quantification of the level of AtRGS1-YFP protein following normalization to the expression of plant-actin in the three seedlings. Asterisks indicated significant differences from the seedlings of AtRGS1-YFP at each time point at P < 0.05 or ∗∗P < 0.01.