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. 2017 Jul 12;8:1229. doi: 10.3389/fpls.2017.01229

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Copyright © 2017 Yan, Wang, Fu and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Confocal imaging of endocytosis of AtRGS1 in the plant of AtRGS1-YFP (A), atg2/AtRGS1-YFP (B), atg5/AtRGS1-YFP (C) (normal seedlings, starved seedlings stimulated by 6% D-glucose for 0 and 0.5 h). Scale bars represent 10 μm. (D) Quantification of AtRGS1-YFP internalization rate. Internalization rate was calculated by dividing the internalization of the starved seedlings treated with D-glucose for 0 or 0.5 h by the internalization of the normal seedlings. Experiments were performed three independent replicates with similar results. Asterisks, significant differences from the starved seedlings treated with D-glucose for 0 h, ^∗∗P < 0.01.