Table 4.
Probe | Gene | Relation to gene region | DNA methylation | Gene expression | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|
IS average | IR − IS | p value | T2Db,c [10] | BMIb,d [20] | GBPb,e [12] | IS average | IR/IS | p value | |||
cg07251857 | ALPK3 | 1st exon | 0.546 | 0.026 | 0.022 | 0.016 | 76 | 0.89 | 2.56 × 10−3 | ||
cg06532379 | ALPK3 | 1st exon | 0.193 | 0.039 | 0.015 | 0.015 | 76 | 0.89 | 2.56 × 10−3 | ||
cg14080050 | B4GALT1 | Body | 0.447 | −0.037 | 0.015 | −0.014 | 228 | 1.10 | 1.48 × 10−3 | ||
cg13858803 | B4GALT1 | Body | 0.566 | 0.027 | 0.040 | 0.027 | 228 | 1.10 | 1.48 × 10−3 | ||
cg00300298 | BCL2L1 | Body | 0.251 | −0.037 | 0.038 | −0.019 | 151 | 1.07 | 2.01 × 10−3 | ||
cg12873919 | BCL2L1 | Body | 0.504 | −0.036 | 0.032 | 151 | 1.07 | 2.01 × 10−3 | |||
cg03290977 | C1QTNF7 | Body | 0.247 | −0.035 | 0.034 | −0.024 | 44 | 0.86 | 3.28x10−3 | ||
cg01939704 | C1QTNF7 | Body | 0.616 | 0.020 | 0.022 | 44 | 0.86 | 3.28 × 10−3 | |||
cg07538039 | C1QTNF7 | Body | 0.610 | 0.025 | 0.021 | 44 | 0.86 | 3.28 × 10−3 | |||
cg06097727 | C1QTNF7 | Body | 0.547 | 0.035 | 0.043 | 0.016 | 44 | 0.86 | 3.28 × 10−3 | ||
cg24829483 | C1QTNF7 | 5′UTR | 0.633 | 0.039 | 0.034 | 44 | 0.86 | 3.28 × 10−3 | |||
cg00545229 | C1QTNF7 | TSS200 | 0.563 | 0.041 | 0.018 | 44 | 0.86 | 3.28 × 10−3 | |||
cg15372098 | C3orf26 | Body | 0.027 | −0.016 | 0.014 | 69 | 0.92 | 3.98 × 10−4 | |||
cg00991994 | C3orf26 | Body | 0.401 | 0.055 | 0.039 | 0.067 | 0.035 | 69 | 0.92 | 3.98 × 10−4 | |
cg17351376 | CD248 | 1st exon | 0.504 | 0.019 | 0.032 | 239 | 1.42 | 1.03 × 10−3 | |||
cg07145284 | CD248 | TSS200 | 0.085 | 0.029 | 0.038 | 0.018 | 239 | 1.42 | 1.03 × 10−3 | ||
cg00350296 | CD248 | TSS1500 | 0.158 | 0.041 | 0.018 | 0.022 | 239 | 1.42 | 1.03 × 10−3 | ||
cg13860849 | CD248 | 1st exon | 0.191 | 0.054 | 0.002 | 0.015 | 239 | 1.42 | 1.03 × 10−3 | ||
cg10772263 | CHST3 | 5′UTR | 0.322 | 0.020 | 0.028 | 0.025 | 113 | 1.17 | 1.31 × 10−3 | ||
cg04268405 | CHST3 | TSS1500 | 0.369 | 0.042 | 0.024 | 0.219 | 113 | 1.17 | 1.31 × 10−3 | ||
cg12081643 | COL4A1 | 3′UTR | 0.670 | −0.042 | 0.008 | 530 | 1.17 | 1.34 × 10−3 | |||
cg20818806 | COL4A1 | Body | 0.299 | 0.042 | 0.019 | 530 | 1.17 | 1.34 × 10−3 | |||
cg02658690 | COL4A1 | Body | 0.207 | 0.042 | 0.014 | 0.218 | 530 | 1.17 | 1.34 × 10−3 | ||
cg10908116 | COL4A1 | Body | 0.247 | 0.043 | 0.017 | 0.053 | 0.026 | 530 | 1.17 | 1.34 × 10−3 | |
cg02099572 | COL4A1 | Body | 0.140 | 0.047 | 0.005 | 0.056 | 530 | 1.17 | 1.34 × 10−3 | ||
cg03430597 | COL5A1 | Body | 0.751 | 0.018 | 0.004 | 0.018 | 162 | 1.10 | 5.97 × 10−4 | ||
cg24354213 | COL5A1 | Body | 0.601 | 0.027 | 0.023 | 0.014 | 162 | 1.10 | 5.97 × 10−4 | ||
cg14274542 | COL5A1 | Body | 0.596 | 0.037 | 0.019 | 0.012 | 162 | 1.10 | 5.97 × 10−4 | ||
cg10765212 | COL5A2 | TSS200 | 0.129 | 0.021 | 0.047 | 246 | 1.20 | 3.25 × 10−4 | |||
cg15194531 | FMNL1 | Body | 0.466 | 0.041 | 0.005 | 0.018 | 165 | 1.09 | 5.32 × 10−4 | ||
cg08145262 | FRS2 | 5′UTR | 0.658 | 0.031 | 0.020 | 0.020 | 155 | 0.93 | 1.64 × 10−3 | ||
cg19563525 | FRS2 | 5′UTR | 0.382 | 0.035 | 0.006 | 0.017 | 155 | 0.93 | 1.64 × 10−3 | ||
cg10227830 | GAB1 | Body | 0.272 | 0.039 | 0.016 | 141 | 0.89 | 1.53 × 10−4 | |||
cg25911551 | GAB1 | Body | 0.494 | 0.046 | 0.049 | 0.019 | 141 | 0.89 | 1.53 × 10−4 | ||
cg08202226 | GATAD2B | TSS1500 | 0.793 | −0.057 | 0.018 | −0.029 | 282 | 0.94 | 3.97 × 10−3 | ||
cg05514401 | IRS2 | 1st exon | 0.792 | 0.031 | 0.002 | 0.028 | 242 | 0.85 | 1.24 × 10−3 | ||
cg11624345 | KCNN4 | Body | 0.391 | 0.025 | 0.025 | 0.014 | 87 | 1.06 | 4.11 × 10−3 | ||
cg03731131 | KCNN4 | Body | 0.378 | 0.032 | 0.039 | 87 | 1.06 | 4.11 × 10−3 | |||
cg22904711 | KCNN4 | Body | 0.313 | 0.060 | 0.002 | 0.047 | 0.015 | 87 | 1.06 | 4.11 × 10−3 | |
cg14616541 | MYH10 | Body | 0.834 | 0.024 | 0.010 | 292 | 0.87 | 1.64 × 10−3 | |||
cg22588546 | MYH10 | Body | 0.496 | 0.047 | 0.008 | 0.039 | 292 | 0.87 | 1.64 × 10−3 | ||
cg21542094 | PFKFB3 | TSS1500 | 0.081 | −0.001 | 0.025 | −0.013 | 542 | 0.80 | 5.53 × 10−5 | ||
cg00902516 | PFKFB3 | Body | 0.739 | 0.020 | 0.019 | 0.016 | 542 | 0.80 | 5.53 × 10−5 | ||
cg03261682 | PFKFB3 | Body | 0.780 | 0.028 | 0.006 | 0.026 | 542 | 0.80 | 5.53 × 10−5 | ||
cg05686026 | PFKFB3 | Body | 0.683 | 0.045 | 0.001 | 0.033 | 542 | 0.80 | 5.53 × 10−5 | ||
cg03478610 | PPP2R3A | 5′UTR | 0.871 | −0.031 | 0.034 | −0.014 | 91 | 0.93 | 1.49 × 10−3 | ||
cg00369142 | PPP2R3A | 3′UTR | 0.378 | 0.044 | 0.013 | 0.060 | 0.025 | 91 | 0.93 | 1.49 × 10−3 | |
cg11468953 | PTPRJ | Body | 0.519 | −0.039 | 0.027 | −0.020 | 139 | 1.18 | 3.07 × 10−3 | ||
cg12124589 | QSOX1 | Body | 0.775 | −0.032 | 0.027 | −0.020 | 175 | 1.08 | 1.70 × 10−3 | ||
cg09505809 | QSOX1 | TSS1500 | 0.179 | 0.039 | 0.031 | 175 | 1.08 | 1.70 × 10−3 | |||
cg00971364 | RBMS3 | TSS200 | 0.043 | −0.018 | 0.034 | 381 | 0.90 | 5.14 × 10−4 | |||
cg23537305 | RBMS3 | Body | 0.819 | 0.016 | 0.045 | 0.017 | 381 | 0.90 | 5.14 × 10−4 | ||
cg20299414 | RBMS3 | Body | 0.729 | 0.035 | 0.018 | 0.013 | 381 | 0.90 | 5.14 × 10−4 | ||
cg27569887 | RBMS3 | 3′UTR | 0.698 | 0.043 | 0.026 | 381 | 0.90 | 5.14 × 10−4 | |||
cg16572224 | SH3PXD2B | Body | 0.816 | −0.049 | 0.002 | −0.039 | −0.019 | 145 | 1.13 | 4.76 × 10−3 | |
cg05223396 | SH3PXD2B | Body | 0.404 | 0.025 | 0.049 | 145 | 1.13 | 4.76 × 10−3 | |||
cg09744420 | STX11 | Body | 0.654 | 0.041 | 0.002 | 0.020 | 356 | 0.89 | 2.98 × 10−3 | ||
cg19841369 | SYNE2 | Body | 0.159 | 0.028 | 0.044 | 0.016 | 236 | 0.89 | 2.18 × 10−3 | ||
cg16725974 | SYNE2 | 5′UTR | 0.532 | 0.046 | 0.027 | 0.057 | 0.022 | 236 | 0.89 | 2.18 × 10−3 | |
cg23250157 | SYNE2 | Body | 0.756 | 0.061 | 0.018 | 236 | 0.89 | 2.18 × 10−3 | |||
cg18837713 | ZDHHC17 | Body | 0.616 | 0.045 | 0.010 | 0.027 | 161 | 0.94 | 5.15 × 10−3 |
aDifferentially expressed genes (10% FDR) accompanied by DMS (p < 0.05) in SAT between insulin-resistant and insulin-sensitive women. Groups were compared using Limma and adjusting for BMI (gene expression, DMS) and age (DMS). This table contains a subset of the ESM Table 4 and focuses on DMS confirmed from the literature and mentioned in the discussion
bComparison with published transcriptome profiles on SAT from insulin-resistant vs insulin-sensitive individuals
cT2D vs control
dRegression coefficient
eAfter vs before bariatric surgery and weight loss
IR, insulin-resistant; IS, insulin-sensitive; T2D, type 2 diabetes