Fig. 1.

eNAMPT-monomer levels are selectively elevated in HFD-fed mice. (a) Total serum eNAMPT, (b, c) serum eNAMPT monomeric and dimeric protein and (d) serum NMN levels in CON- or HFD-fed mice (fed for 10 weeks); in (c) white bars, CON-fed; black bars, HFD-fed. (e) Nampt mRNA expression (normalised to control levels [levels in CON-fed mice]), (f, g) NAMPT-monomer and NAMPT-dimer protein levels (normalised to control [CON-fed]) and (h) total NAD levels in white AT (in (c) and (g), white bars, CON-fed; black bars, HFD-fed). eNAMPT secretion from (i) SVF and (j) white adipocytes. Western blots are representative of three separate blots. *p < 0.05, **p < 0.01