Fig. 3.

eNAMPT immunoneutralisation reverses beta cell dysfunction in HFD-fed mice. (a) Ex vivo GSIS and (b, c) AUC insulin response to IPGTT in CON- and HFD-fed mice (fed for 10 weeks) i.p. injected with eNAMPT-Ab or non-immune IgG. (d) Insulin immunofluorescence staining of whole pancreatic sections (magnification ×20), (e) relative islet size (AU, arbitrary unit) and (f) gene expression of pro-apoptotic genes in isolated islets (normalised to control [CON^IgG]). (a, f) white, CON^IgG; light grey, CON^Ab; black, HFD^IgG; dark grey, HFD^Ab. *p < 0.05, ** p < 0.01 for all subparts except (f) where ^††† p < 0.001 for CON^IgG vs HFD^IgG, ^‡‡‡ p < 0.001 for HFD^IgG vs HFD^Ab and ^§§ p < 0.01 for CON^Ab vs HFD^IgG