$\dot{V}$O2 (ml.kg.min−1)	0 min	5 min	10 min	15 min	20 min	25 min	30 min
TPC1TEMPFLAT	6.4 ± 1.8	34.3 ± 4.2*	35.1 ± 5.6*	36.2 ± 6.4*	36.1 ± 5.6*	35.9 ± 5.0*	36.0 ± 4.0*
TPC2HOTDOWN	6.0 ± 1.0	34.2 ± 6.2*	35.1 ± 6.5*	36.1 ± 7.8*	36.7 ± 7.8*	36.1 ± 6.8*	36.2 ± 7.2*
HPC1HOTDOWN	6.3 ± 1.5	32.0 ± 1.8*	32.6 ± 2.1*	34.4 ± 1.7*	35.6 ± 1.4*	36.7 ± 1.8*	37.2 ± 1.7*
HPC2HOTDOWN	6.5 ± 0.8	32.0 ± 1.3*	33.4 ± 1.6*	33.6 ± 2.5*	35.4 ± 2.4*	36.0 ± 2.7*	36.4 ± 2.8*
RPE (Units)
TPC1TEMPFLAT	6.0 ± 0.0	10.0 ± 2.0	10.4 ± 0.8	11.8 ± 1.5	12.6 ± 1.5	13.2 ± 1.6	13.4 ± 1.6
TPC2HOTDOWN	6.0 ± 0.0	11.4 ± 1.3C	13.4 ± 0.9C	14.4 ± 0.6C	15.4 ± 0.9C	16.0 ± 0.8C	17.3 ± 1.0C
HPC1HOTDOWN	6.0 ± 0.0	11.5 ± 1.1C	13.2 ± 0.8C	14.2 ± 1.1C	15.3 ± 0.5C	16.5 ± 0.6C	17.5 ± 1.2C
HPC2HOTDOWN	6.0 ± 0.0	11.8 ± 0.8	12.8 ± 0.8	13.7 ± 1.0	15.1 ± 1.0	16.0 ± 1.3	17.0 ± 1.3
TS (Units)
TPC1TEMPFLAT	4.0 ± 0.0	3.6 ± 1.1	4.4 ± 1.0	5.0 ± 0.9	5.4 ± 0.7	5.5 ± 0.6	5.5 ± 0.7
TPC2HOTDOWN	4.0 ± 0.0	5.1 ± 0.7C	5.6 ± 0.7C	6.0 ± 0.7C	6.6 ± 0.7C	7.0 ± 0.4C	7.3 ± 0.3C
HPC1HOTDOWN	4.0 ± 0.0	4.7 ± 0.5C	5.3 ± 0.4C	5.8 ± 0.5C	6.3 ± 0.4C	6.8 ± 0.4C	6.9 ± 0.4C
HPC2HOTDOWN	4.0 ± 0.0	4.8 ± 0.4	5.5 ± 0.7	5.7 ± 0.7	6.3 ± 0.4	6.7 ± 0.4	6.8 ± 0.4

Values are expressed as mean ± SD for quadriceps tenderness and $\dot{V}$O₂. Values are expressed as median ± IQR for B[La], RPE, TS and Urine Osmolality.

^*Increased compared to basal (p < 0.05).

^AIncreased compared to HPC1_HOTDOWN.

^BIncreased from basal (main effect).

^CIncreased compared to TPC1_TEMPFLAT.