Table 2.
Primers used in this study.
| Primer description | Sequence 5′-3′ | Tannaeling [°C] | Product length [bp] |
|---|---|---|---|
| Quantitative reverse transcriptase PCR | |||
| cbbL_810F 5′ | AGGTCTTGCGAACTACTGTC | 51 | 306 |
| cbbL_1115R | CCAGAAGCAACTGGCATAAC | ||
| cbbM_647F | TCTGCACGGTAGCACATTTC | 51 | 330 |
| cbbM_976R | ATTTGACGGTCCTGCTGTTG | ||
| ChlR_821F | TAAGCATTCTGCCGACATGG | 51 | 284 |
| ChlR_1104R | CGATTTCCGGCAGTTTCTAC | ||
| rpoD_416F | GATCAACGACATGGGCATTC | 51 | 305 |
| rpoD_720R | CGTACTGTTCCAGCAGATAG | ||
| 16S_280F | GGTCGCTTCTCTTTGTATGC | 51 | 285 |
| 16S_564R | CCTTACCTGGTCTTGACATC | ||
| cbbO-m_700F | ACCTCATCGCCATAATGCTC | 51 | 223 |
| cbbO-m_922R | TTGCCGTCATGTTACTGGTC | ||
| cbbQ-m_2828F | GTGGCGGCATACACCATTAG | 51 | 147 |
| cbbQ-m_2974R | AGTCGAAGCGCACATCTTAC | ||
| cbbQ-1_9714F | GGTAGGTCGCTTCCTAATCG | 51 | 199 |
| cbbQ-1_9912R | GTGCGCTTCTACCAACTCAC | ||
| cbbO-1_12263F | CGCATGCCATCAATGGTATC | 51 | 263 |
| cbbO-1_12525R | GTCAATATCCGCTGGTTCAC | ||
| Protein expression | |||
| CbbLF_NheI | GCTAGCACCATGGCTAAGACTTATAAC | 501/602 | 1421 |
| CbbLR_BamHI | GGATCCGCCTTATGCTTAACATCTAGCTTAT | ||
| CbbMF_NheI | GCTAGCATGGATCAGTCGAATCGTTATG | 561/622 | 1386 |
| CbbMR_BamHI | GGATCCGCTTTGTGTACGCCCAACTTCTC | ||
| LysR1F_NheI | GCTAGCATGCλCTTACATATAACCGCCCAGC | 621/672 | 932 |
| LysR1R_BamHI | GGATCCGAGCGCGTGTCCGACATGG | ||
| LysR2F_NheI | GCTAGCATGCCTGλAATTTCCATCC | 511/612 | 950 |
| LysR2R_BamHI | GGATCCGAGCGAAATTGGTTAAACG | ||
| Preparation of Cy3-labeled DNA fragments | |||
| ncrQm-M_3427F | AGCCGCTTCATAAAGTTC | 56.3 | 371 |
| ncrQm-M_3797R | CAGATGCAGACACAATCTAC | ||
| ncrM-R2_4993F | TGCAGCAACTTCTAAGTAACC | 62.2 | 369 |
| ncrM-R2_5361R | ACAGGCGTTTAACCAATTTCG | ||
| ncrR2 L_7191F | CTGGGCGGTTATATGTAAG | 57.7 | 275 |
| ncrR2-L_7465R | TACACCGGCGTTATAAGTC | ||
| ncrS-Q1_9151F | AACCACGTTCGTTTGATTG | 57.5 | 331 |
| ncrS-Q1_9531R | TGGCTCGTCTTTAATAAGG | ||
1For the first 5 cycles/2 for 25 additional cycles; Tannaeling, annealing temperature.