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. 2017 Jan 18;178(2):327–337. doi: 10.1007/s12011-017-0931-4

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© The Author(s) 2017

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 4 — a–g Intensity of ROS production in mitochondria. Images from a confocal microscope (FV1000 (Olympus, Germany) showing hepatocytes incubated with the probe mitochondrial MitoSOX, after 48 h cultivation of cells in a medium containing an extract of Japanese quince. a Control. b Positive control. c 1 μg/mL. d 10 μg/mL. e 50 μg/mL. f 100 μg/mL. g Positive control for enhanced ROS formation—hepatocytes cultured in a medium supplemented with 10 μM of ethyl alcohol. Pictures were taken using a HeNe-G helium neon laser (575/625 nm wavelength—red fluorescence) (color figure online)