Fig. 3.

Graph showing same simulated example as in Fig. 2 (left panel), except that three variants are positively orientated and two negatively. The inverse-variance weighted estimate (solid line) is unaffected by the orientation of variants, whereas the MR-Egger estimate (dashed line) is affected by the choice of orientation, with the intercept term attenuating and the MR-Egger estimate approaching the inverse-variance weighted estimate