Skip to main content
. Author manuscript; available in PMC: 2017 Jul 12.
Published in final edited form as: Biol Cell. 2017 Mar 7;109(4):162–166. doi: 10.1111/boc.201600064

Figure 1. Arpin depletion does not impair chemotaxis in MDA-MB-231 cells.

Figure 1

Control MDA-MB-231 clones (shRNA Ctrl #1 and #2) or Arpin-depleted clones (shRNA Arpin #1 and #2) were seeded in the left chamber of the microfluidic device, whereas chemoattractant containing medium was placed on the right chamber of the microfluidic device. Two symmetrical devices are shown: they both display a bifurcation, where cells choose either a short or a long path. The short path has a steeper gradient than the long one. A chemotactic cell thus chooses the short path. Depending on the device, the short path is straight ahead (right histogram) or at an angle (left histogram). In both cases, Arpin-depleted clones do not display a chemotaxis behaviour that differs from the one of control clones. A single experiment is displayed. The cells were starved for 5 h and chemotaxis was stimulated in the microfluidic device by 1% foetal calf serum and 20 ng/ml EGF. The number of cells counted in each condition is indicated below the histograms.