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. 2017 May 18;151(4):405–416. doi: 10.1111/imm.12738

Figure 4.

Figure 4

Identification of regulatory T (Treg) cell subsets in antiretroviral‐naive HIV infection using CD45RA, CD27, CD62L and CCR7 cell surface markers. (a) Representative dot plots of the gating strategy of different Treg cell subsets. Purified Treg cells obtained from peripheral blood mononuclear cells (PBMCs) of HIV + (n = 31) and HIV – (n = 17) participants were stained using monoclonal antibodies specific for CD3, CD4, CD25, CD127, CD45RA, CD27, CD62L and CCR7 and then analysed by multiparametric flow cytometry. Dot plots showing co‐expression of both CD27 and CD45RA on CD4+ CD25+ CD127lo Treg cells are represented in the middle panel. The data on CCR7 and CD62L are shown in each CD27 CD45RA subset. Distribution of Treg cell subsets analysed on CD45RA + or CD45RA – Treg cells are shown in (b) and (c), respectively. Whereas seronegative people displayed predominantly naive (P < 0·05) and central memory (P < 0·001) phenotypes, antiretroviral therapy‐naive HIV‐infected participants showed significantly elevated numbers of effector (P < 0·001) and effector memory (P < 0·05) Treg cell subsets. Bars represent the median and Mann–Whitney U‐test was used to compare each subset between HIV‐infected and uninfected controls. *P<0·05; **P<0·001.