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. 2017 May 30;16(4):814–824. doi: 10.1111/acel.12619

Figure 6.

Figure 6

CEH‐23, CEP‐1 likely act downstream of CRTC‐1. (A) More CRTC‐1 was excluded from the nucleus in the isp‐1 mutant compared to wild‐type worms, and the nuclear exclusion was slightly enhanced when ceh‐23 and cep‐1 were mutated. CRTC‐1 localization experiments were performed using 40 young adults (YA) worms for each genotypes and the localization of CRTC‐1 in intestinal cells was scored. Microscopic images show representative nuclear‐excluded and nuclear‐localized CRTC‐1 pattern. Gray arrows denote intestinal nuclei (P‐value: ****< 0.0001, ***< 0.0005, *< 0.05). (B, C) Forced expression of constitutively nuclear CRTC‐1 partially suppressed the prolonged lifespan of the isp‐1 mutant and ceh‐23 (B) or cep‐1 (C) RNAi knockdown did not further impact the suppressed lifespan.