Figure 4.

The inflammatory cell infiltration was significantly decreased in the colon tissues of ficolin‐A (FCN‐A) knockout (KO) mice. Flow cytometry analysis of the percentages of (a) CD45⁺ leucocytes, (b) CD11b⁺ F4/80⁺ macrophages, (c) CD11b⁺ Gr1⁺ neutrophils and (d) CD11b⁺ CD11c⁺ dendritic cells in the colon tissues of each group of mice. (e) IHC analysis of the colon tissues with an anti‐CD68 for determining the presence of macrophages (arrows, brown). (f) Quantitative analysis of CD68 staining by image pro plus 6·0 software. Six mice were analysed in each group. Values are mean ± SEM from three independent experiments. *P < 0·05, ns: not significant.