Skip to main content
. 2017 Aug;45(8):887–895. doi: 10.1124/dmd.117.075861

Fig. 2.

Fig. 2.

Comparison of the short- and long-term effects of PKC on hOAT1 expression at the cell surface and in total cell lysates in HeLa cells. (A) hOAT1 expression at the cell surface. hOAT1-expressing cells were treated with or without PKC activator PMA for 30 minutes and 4 hours, respectively. Cell surface biotinylation was then performed. Biotinylated/cell surface proteins were separated with streptavidin beads and analyzed by immunoblotting (IB) with an anti-myc antibody (myc was tagged to hOAT1 for immuno-detection). (B) Densitometry plot of results from (A) as well as from other independent experiments. The values are mean ± S.E. (n = 3). *P < 0.05. (C) hOAT1 expression in total cell lysate. hOAT1-expressing cells were treated with or without PKC activator PMA for 30 minutes and 4 hours, respectively. hOAT1 total expression was analyzed by IB with an anti-myc antibody. (D) Densitometry plot of results from (C, top panel) as well as from other independent experiments. The values are mean ± S.E. (n = 3). *P < 0.05.