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. 2017 Aug;45(8):887–895. doi: 10.1124/dmd.117.075861

Fig. 7.

Fig. 7.

Effect of Nedd4-2 WW domain mutant Mut-WW3/WW4 on hOAT1 ubiquitination. (A, top panel) hOAT1-expressing COS-7 cells were transfected with cDNAs for wild-type Nedd4-2 or for Nedd4-2 WW domain mutant (Mut-WW3/WW4). Transfected cells were then lysed, and hOAT1 was immunoprecipitated (IP) with anti-myc antibody, followed by immunoblotting (IB) with anti-ubiquitin antibody (anti-Ub). (Bottom panel) The blot from the top panel was reprobed with anti-myc antibody to determine the amount of hOAT1 IP. (B) Densitometry plot of results from (A, top panel) as well as from other independent experiments. The values are mean ± S.E. (n = 3). *P < 0.05.