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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Biomaterials. 2017 Apr 22;134:22–30. doi: 10.1016/j.biomaterials.2017.04.039

Figure 6.

Figure 6

ATC rescues YAP activity in hMSCs seeded on soft substrates or with small cell areas. (A&B) Representative fluorescence images (A) and bar plot (B) showing subcellular localization of YAP in hMSCs on the PDMS micropost arrays (PMAs) with different rigidities as indicated. hMSCs were loaded with functionalized microbubbles (MBs) before ultrasound (US) stimulation (+US +MB). Negative control of hMSCs without MBs and US stimulation (−US −MB) was included for comparison. White dashed lines in micrographs highlight cell boundaries. Scale bar in A, 50 μm. (C&D) Representative fluorescence images (C) and bar plot (D) showing subcellular YAP localization in micropatterned hMSCs with different cell areas as indicated. White rectangles in micrographs highlight cell boundaries. Scale bar in C, 50 μm. hMSCs were fixed and stained for YAP 30 min after US stimulation. Error bar denotes SEM. with n ≥ 3. *, P < 0.05; **, P < 0.01. US parameter: frequency 1 MHz, pulse repetition frequency 10 Hz, pulse duration 50 ms, treatment duration 5 min, and acoustic pressure 0.08 MPa.