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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Methods Mol Biol. 2017;1486:483–509. doi: 10.1007/978-1-4939-6421-5_19

Figure 2.

Figure 2

Preparation of flow chambers for optical trapping. (a) Beads in nitrocellulose are first added to one side of a clean cover glass in a straight line. A pipet tip is then used to spread the beads across the surface of the glass. Bead-coated cover glasses are dried, wet side up, in petri dishes. Pipet tips are used to elevate the cover glass (visible in 2c). (b) Fully assembled chambers are formed using double-stick tape and vacuum grease. (c) Flow chambers are filled by adding liquids to chambers that are raised on petri dishes. Liquid that has flowed through the chamber can be collected using cotton-tipped applicators or filter paper.