Sequence alignment of PECACE domains identified in Gram-positive bacteria. Multiple sequence alignment was constructed using ClustalW. The lengths of the insertions in the sequences are shown in parentheses. The sequences are denoted by their GenBank Identifier (gi). The domain limits are indicated by the residue positions (first-end). The amino acids identified as catalytic or involved in ligand recognition are marked with asterisks under PECACE sequence. Alignments are coloured using the CHROMA tool using default parameters [40]. Full sequence details, group (i): Streptococcus pneumoniae R6 (gi:15902974), Streptococcus mitis NCTC 12261 (§SMT1418), Streptococcus sanguinis SK36 (&:SS_A352_G10), Streptococcus gordonii (gi:18389219), Streptococcus suis P1/7 (suis166b12), Streptococcus uberis 0140J (sub49a04), Streptococcus equi (equi324d3), Streptococcus equi subsp. Zooepidemicus (zoo26g07), Streptococcus pyogenes M1 GAS (gi:15675124), Streptococcus agalactiae 2603V/R (gi:22537230), Lactococcus lactis subsp. Cremoris SK11 (scaffold18), Streptococcus mutans UA159 (gi:24379517), Streptococcus thermophilus LMD-9 (scaffold3), Lactococcus lactis subsp. Lactis (gi:15672584), Enterococcus faecium DO (2351355_Cont543), Enterococcus faecalis V583 (gi:29376084), Bacillus subtilis subsp. subtilis str. 168 (gi:16078973), Bacillus cereus ATCC 14579 (gi:30020591), Oceanobacillus iheyensis HTE831 (gi:23100516), group (ii): Bacillus anthracis: (pXO2-08) (gi:10956398), Enterococcus faecalis: (pRE25) (gi:12957015), Enterococcus faecium (gi:22992993), Enterococcus faecalis V583 (gi:29376781), Clostridium difficile 630 (Cd81d2), Enterococcus faecalis V583 (gi:29376405), Clostridium perfringens (gi:13274506), Staphylococcus aureus subsp. aureus Mu50 (gi:15923390), Listeria monocytogenes EGD-e (gi:16803144), Streptococcus agalactiae 2603V/R (gi:22537089), Enterococcus faecium (gi:22993467), Bacillus subtilis subsp. subtilis str. 168 (gi:16077564, group (iii): Bacillus cereus ATCC 14579 (gi:30021796), group (iv): Enterococcus faecalis BM4518 (gi:33355845), group (v): Bacillus anthracis str. A2012: (pXO1) (gi:21392795), Bacillus cereus ATCC 10987: (pBc10987) (gi:44004362).