FIG 3 .
VSG trafficking is TbERP dependent. Specific dsRNA was induced for 72 h in each of the BSF TbERP RNAi cell lines as indicated. Control (Tet-, open circles) and induced (Tet+, closed circles) cells were pulse (2 min)-chase (60 min) radiolabeled, and VSG trafficking was analyzed over time by hypotonic lysis as described in Materials and Methods. VSG was specifically immunoprecipitated from cell fractions and analyzed by SDS-PAGE and phosphorimaging (2 × 106 cell equivalents per lane). (Top) The means ± SEM from biological triplicate experiments are presented as the loss of cell-associated VSG normalized to T0. (Bottom) Representative phosphorimages of cell-associated VSG.