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. 2017 Jul 12;5:74. doi: 10.1186/s40168-017-0291-5

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Experimental challenge timeline (a). Schematic of challenge and treatment in each quarter (b). Effect of intramammary infection with E. coli and treatment with ceftiofur hydrochloride (48, 72, 96, 120, and 144 h) on temperature (c). California Mastitis Test (CMT) results (d). RAPD strain typing results. (e) Effect of intramammary infection with E. coli and treatment with ceftiofur hydrochloride (48, 72, 96, 120, and 144 h) on colony-forming units (CFU) (f) Asterisks represent differences after Tukey-Kramer multiple comparison correction and α=0.05 between groups within the same time point