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Primer sequences used for PCR amplification
| Gene | Primer sequence (5′–3′) |
|---|---|
|
AGTR1
(122 bp, NM_032049.3) |
Forward: GGAAACAGCTTGGTGGTGAT |
| Reverse: GCCCATAGTGGCAAAGTCA | |
|
AGTR2
(100 bp, NM_000686.4) |
Forward: ACATCTTCAACCTCGCTGTG |
| Reverse: ACAGGTCCAAAGAGCCAGTC | |
|
GAPDH
(138 bp, NM_002046) |
Forward: CAGGAGGCATTGCTGATGAT |
| Reverse: GAAGGCTGGGGCTCATTT |
