Table 1.

Source of the immunoenzymatic assay kits, sensitivity, precision, and reference values from healthy individuals.

Marker	Commercial name (source)	Detection limit	Intraassay CV Min–max (%)	Interassay CV Min–max (%)	Female (postmenopausal) Mean ± SEM Median Min–max	Female (premenopausal) Mean ± SEM Median Min–max	Male Mean ± SEM Median Min–max
BAP (μg/l)	Ostase BAP (Immunodiagnostic systems)	0.7	2.6–6.5	3.7–6.1	14.53 ± 4.21	9.0 ± 2.98	13.13 ± 3.8
					13.2	8.78	12.3
					8.0–22.4	4.0–14.30	7.0–20.1
CTX (μg/l)	Serum crosslaps (Immunodiagnostic systems)	0.02	1.8–3.0	2.5–10.9	0.64 ± 0.36	0.38 ± 0.19	0.39 ± 0.19
					0.44	0.29	0.29
					0.12–1.35	0.11–0.74	0.12–0.75
int-OC (μg/l)	hOST EASIA (DIAsource ImmunoAssays)	0.08	3.1–4.7	3.5–5.6	15.00 ± 5.77
					15.00
					5.0–25.0
N-mid OC (μg/l)	N-mid Osteocalcin (Immunodiagnostic systems)	0.5	1.3–2.2	4.2–5.1	31.43 ± 12.43	19.90 ± 7.47	23.40 ± 9.19
					26.5	17.40	19.8
					12.8–55	8.40–33.9	9.6–40.8
OPG (pmol/l)	Osteoprotegerin (Biomedica)	0.07	2-3	3–5	—
					2.7
					—
PICP (μg/l)	MicroVue CICP EIA (Quidel)	0.2	5.0–7.2	Not declared	116.00 ± 27.14
					116
					69.0–163
RANKL (pg/l)	Free soluble RANKL (high sensitivity) (Biomedica)	0.01	≤5%	≤3%	—
					0.14
					—

BAP: bone alkaline phosphatase; CTX: cross-linked C-telopeptide of type I collagen; int-OC: intact osteocalcin; N-mid OC: N-terminal/midregion osteocalcin; OPG: osteoprotegerin; PICP: C-terminal propeptide of type I procollagen; RANKL: receptor activator of nuclear factor kB ligand.