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. 2017 Jul 11;4:17. doi: 10.1186/s40662-017-0082-x

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — MCP-1 transcript and protein expression in HCE-T cells. a qPCR results of MCP-1 transcript levels in control HCE-T cells, and in HCE-T cells transfected with pSEAP-basic or pSEAP-NF-κB, either stimulated or un-stimulated with Pam3CSK4, treated or un-treated with IκK inhibitors, Wedelolactone and BMS-345541. GAPDH was used as the housekeeping control. b MCP-1 protein concentration in various cell culture media. Cell culture media were collected from cells transfected with pSEAP-basic or pSEAP-NF-κB with various treatment. ELISA was used to determine MCP-1 protein concentration. The means of two independent experiments are shown. Error bars represent standard error of the means. *: P < 0.05