Lung cells were stained with CD11c, CD11b, F4/80, Ly6G, MHCII, CD86 and CD206 for immunophenotyping. A) Activated double positive (DP) macrophages - CD11c+CD11b+F4/80+Ly6G 3 days following BHT or oil ; B) Additional markers for DP activated macrophages at the 3 day time point - MHCII+CD86+ or CD206+; C) Activated double positive (DP) macrophages - CD11c+CD11b+F4/80+Ly6G 6 days following BHT or oil ; D) Additional markers for DP activated macrophages at the 6 day time point - MHCII+CD86+ or CD206+;Dendritic cells- CD11c+CD11b+F4/80Ly6G 3 (E) and 6 (F) days following BHT or oil. Mean ± SEM presented; n = 5; repeated 2 times. *, P<0.05 compared to oil controls; +, p<0.05 compared to BALB mice.