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. 2017 Jul 12;8:1277. doi: 10.3389/fmicb.2017.01277

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Copyright © 2017 Moinier, Byrne, Amouric and Bonnefoy.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Binding of 6His-SUMO-RegA, 6His-SUMO-RegA_D68A and 6His-SUMO-RegA_A102S to the regulatory region of target genes involved in Fe(II) and ISC oxidation. EMSA with the regulatory region of the rus, cta, petI, reg, tet, cyo, hdr, doxII, and cyd operons, hdrB and sqr genes as well as with an internal fragment of the 16S rRNA gene from Thiomonas arsenitoxydans (rrs). 6His-SUMO-RegA, 6His-SUMO-RegA_D68A and 6His-SUMO-RegA_A102S concentrations (μM) are indicated above each lane.